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Studies into the microbial spectrum of apical periodontitis
Author(s) -
BRAUNER A. W.,
CONRADS G.
Publication year - 1995
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.1995.tb00308.x
Subject(s) - prevotella intermedia , streptococcus milleri , obligate anaerobe , microbiology and biotechnology , periodontitis , bacteroides , prevotella , biology , streptococcus intermedius , anaerobic bacteria , gram staining , bacteria , root canal , bacteroidaceae , streptococcus , porphyromonas gingivalis , medicine , dentistry , antibiotics , genetics
Summary This study examined the variety of obligate and facultative anaerobic bacterial species recovered from cases of acute apical periodontitis. A total of 19 root canal samples and 24 periapical granuloma samples were taken from patients suffering pain and discomfort. Bacteria were identified by applying the following techniques: culturing on various media, Gram‐staining and using commercially available biochemical test strips. In addition, Prevotella intermedia and Porphyromonas endodontalis were differentiated on a molecular genetic level using species‐specific oligodeoxymicleotide probes. The most frequently identified bacteria were Prevotella intermedia, Bifidobacterium spp., Streptococcus sanguis. Streptococcus milleri‐group and Bacteroides spp. Obligate anaerobes occured at a rate of 82.3%, and the average number of isolates was 6.4 per sample.