Premium
Localization of chondroitin sulphate and dermatan sulphate in human dental pulps — an immunohistochemical study
Author(s) -
BARTOLD P. M.,
SCHLAGENHAUF U.
Publication year - 1995
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.1995.tb00151.x
Subject(s) - immunoperoxidase , chondroitin , glycosaminoglycan , chemistry , chondroitin sulphate , monoclonal antibody , connective tissue , immunohistochemistry , dermatan sulfate , biochemistry , chondroitin sulfate , microbiology and biotechnology , antibody , pathology , biology , immunology , medicine
Summary The distribution of dermatan sulphate and chondroitin sulphate in human dental pulps has been assessed using monoclonal antibodies and immunoperoxidase localization techniques. The pulpal tissues were reacted with specific antibodies following pretreatment of the sections with chondroitinase ACII or chondroitinase ABC. Both the 4‐ and 6‐sulphated isomers of chondroitin sulphate were detected in the tissues studied. Very little derrmatan sulphate could be detected. These glycosaminoglycans appeared throughout the pulpal connective tissues with a particularly strong localization to the region adjacent to the odontoblastic and predentine layers. Such distribution strongly implicates chondroitin sulphate in the mineralization process of human dentine.