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Cytotoxicity of root canal sealers: a study using HeLa cells and fibroblasts
Author(s) -
KETTERING JAMES D.,
TORABINEJAD MAHMOUD
Publication year - 1984
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.1984.tb00382.x
Subject(s) - hela , root canal , cytotoxicity , fibroblast , cytotoxic t cell , in vitro , toxicity , cell , nucleic acid , microbiology and biotechnology , chemistry , dentistry , biology , biochemistry , medicine , organic chemistry
Summary. The relative cytotoxicity of five root canal sealers was measured by adding various concentrations of freshly mixed and set sealers to cultures of either HeLa or human fibroblast cells. The toxicity was determined by cell survival, which was measured by subsequent incorporation of [ 3 H]‐thymidine into the nucleic acid components of each cell type used. The results showed that all sealers are cytotoxic in both fresh and set states. The ranking of toxicity of sealers on HeLa cells was similar to the one obtained for fibroblasts. Comparison of the results of this study with those of previous investigations questions the value of ranking sealers based only upon their effects in vitro.