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Transcriptome analysis of the citrus red mite, Panonychus citri , and its gene expression by exposure to insecticide/acaricide
Author(s) -
Niu JZ.,
Dou W.,
Ding TB.,
Shen GM.,
Zhang K.,
Smagghe G.,
Wang JJ.
Publication year - 2012
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2012.01148.x
Subject(s) - acaricide , biology , transcriptome , mite , gene , pesticide , pesticide resistance , detoxification (alternative medicine) , insecticide resistance , gene expression , genetics , botany , toxicology , ecology , medicine , alternative medicine , pathology
The citrus red mite, Panonychus citri , is known for its ability rapidly to evolve resistance to insecticides/acaricides and to adapt to hosts that produce toxins. In this study, we constructed an unprecedented four gigabase pair transcriptome of P. citri , which was assembled into 64 149 unique transcripts, the functions of which were annotated by five public databases. A total of 116 unique transcripts were identified as representatives of potential involvement in the detoxification of xenobiotics. Genes recorded to encoding insecticide/acaricide target proteins were also obtained from the P. citri transcriptome. In order to explore novel candidate genes potentially involved in the pesticide detoxification of P. citri , we also constructed digital gene expression libraries of short‐term transcriptome responses of P. citri to pesticides, which resulted in the identification of 120 unique transcripts potentially associated with insecticide/acaricide detoxification. Our study will facilitate molecular research on pesticide resistance in citrus red mites, as well as in other phytophagous mites.