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RNA interference of ace 1 and ace 2 in Chilo suppressalis reveals their different contributions to motor ability and larval growth
Author(s) -
Hui X.M.,
Yang L.W.,
He G.L.,
Yang Q.P.,
Han Z.J.,
Li F.
Publication year - 2011
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2011.01081.x
Subject(s) - chilo suppressalis , biology , rna interference , interference (communication) , larva , rna , microbiology and biotechnology , zoology , gene , ecology , genetics , engineering , channel (broadcasting) , electrical engineering
Acetylcholinesterase (AChE, EC 3.1.1.7) is a key enzyme in terminating synaptic transmission. We knocked down the expression of Csace 1 or Csace 2 using chemically synthesized small interfering RNAs (siRNAs) designed from divergent regions. The mRNA abundance of the two ace genes was reduced to 50–70% of control levels. The enzyme activities were decreased to 40–70%. Silencing of Csace 1 or Csace 2 resulted in a ∼25% mortality rate. Knockdown of Csace 1 had major effects on larval growth inhibition and resulted in reduced larval weight and length, malformation and motor disability, whereas silencing of Csace 2 had only minor effects. These results suggested that both AChE‐1 and AChE‐2 have important roles in maintaining life in this insect and indicated that AChE‐1 might have nontypical functions in regulating larval growth and motor ability.