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Proteomics of cryoprotective dehydration in Megaphorura arctica Tullberg 1876 (Onychiuridae: Collembola)
Author(s) -
Thorne M. A. S.,
Worland M. R.,
Feret R.,
Deery M. J.,
Lilley K. S.,
Clark M. S.
Publication year - 2011
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2010.01062.x
Subject(s) - biology , proteomics , proteome , trehalose , dehydration , gel electrophoresis , biochemistry , metabolism , springtail , microbiology and biotechnology , ecology , gene , ecotoxicology
The Arctic springtail, Megaphorura arctica Tullberg 1876 (Onychiuridae: Collembola), is one of the few organisms known to survive the extreme stresses of its environment by using cryoprotective dehydration. We have undertaken a proteomics study comparing M. arctica, acclimated at −2°C, the temperature known to induce the production of the anhydroprotectant trehalose in this species, and −6°C, the temperature at which trehalose expression plateaus, against control animals acclimated at +5°C. Using difference gel electrophoresis, and liquid chromatography tandem mass spectrometry, we identified three categories of differentially expressed proteins with specific functions, up‐regulated in both the −2°C and −6°C animals, that were involved in metabolism, membrane transport and protein folding. Proteins involved in cytoskeleton organisation were only up‐regulated in the −6°C animals.