z-logo
Premium
Coadaptation of isoacceptor tRNA genes and codon usage bias for translation efficiency in Aedes aegypti and Anopheles gambiae
Author(s) -
Behura S. K.,
Severson D. W.
Publication year - 2011
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2010.01055.x
Subject(s) - biology , anopheles gambiae , aedes aegypti , gene , transfer rna , genetics , codon usage bias , genome , translation (biology) , rna , messenger rna , larva , ecology , malaria , immunology
The transfer RNAs (tRNAs) are essential components of translational machinery. We determined that tRNA isoacceptors (tRNAs with different anticodons but incorporating the same amino acid in protein synthesis) show differential copy number abundance, genomic distribution patterns and sequence evolution between Aedes aegypti and Anopheles gambiae mosquitoes. The tRNA‐Ala genes are present in unusually high copy number in the Ae. aegypti genome but not in An. gambiae . Many of the tRNA‐Ala genes of Ae. aegypti are flanked by a highly conserved sequence that is not observed in An. gambiae . The relative abundance of tRNA isoacceptor genes is correlated with preferred (or optimal) and nonpreferred (or rare) codons for ∼2–4% of the predicted protein coding genes in both species. The majority (∼74–85%) of these genes are related to pathways involved with translation, energy metabolism and carbohydrate metabolism. Our results suggest that these genes and the related pathways may be under translational selection in these mosquitoes.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here