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A novel class of miniature inverted repeat transposable elements (MITEs) that contain hitchhiking (GTCY) n microsatellites
Author(s) -
Coates B. S.,
Kroemer J. A.,
Sumerford D. V.,
Hellmich R. L.
Publication year - 2011
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2010.01046.x
Subject(s) - inverted repeat , transposable element , biology , microsatellite , genome , transposition (logic) , genetics , computational biology , gene , allele , linguistics , philosophy
The movement of miniature inverted repeat transposable elements (MITEs) modifies genome structure and function. We describe the microsatellite‐associated interspersed nuclear element 2 ( MINE ‐2), that integrates at consensus WTTTT target sites, creates dinucleotide TT target site duplications (TSDs), and forms predicted MITE‐like secondary structures; a 5′ subterminal inverted repeat (SIR; AGGGTTCCGTAG) that is partially complementary to a 5′ inverted repeat (IR; ACGAAGCCCT) and 3′‐SIRs (TTACGGAACCCT). A (GTCY) n microsatellite is hitchhiking downstream of conserved 5′ MINE ‐2 secondary structures, causing flanking sequence similarity amongst mobile microsatellite loci. Transfection of insect cell lines indicates that MITE‐like secondary structures are sufficient to mediate genome integration, and provides insight into the transposition mechanism used by MINE ‐2s.