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Validation of novel promoter sequences derived from two endogenous ubiquitin genes in transgenic Aedes aegypti
Author(s) -
Anderson M. A. E.,
Gross T. L.,
Myles K. M.,
Adelman Z. N.
Publication year - 2010
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2010.01005.x
Subject(s) - biology , aedes aegypti , transgene , gene , luciferase , microbiology and biotechnology , vector (molecular biology) , green fluorescent protein , gene expression , transformation (genetics) , genetics , transfection , larva , recombinant dna , botany
To date, only a limited number of promoter sequences have been described to drive transgene expression in the disease vector Aedes aegypti . We sought to increase this repertoire by characterizing the ability of upstream sequences derived from the Ae. aegypti Ub L40 and polyubiquitin genes to drive the expression of marker proteins. Both genomic fragments were able to drive robust expression of luciferase in cultured mosquito cells. Following Mos 1‐transformation, the Ub L40 promoter drove strong expression of a fluorescent marker in early larvae and in ovaries, while the polyubiquitin promoter drove robust EGFP expression in all stages of development, including constitutive expression throughout the midgut. These promoter fragments provide two new expression profiles for future Ae. aegypti genetic experiments.