Premium
Yellow fever mosquito sterol carrier protein ‐2 gene structure and transcriptional regulation
Author(s) -
Vyazunova I.,
Lan Q.
Publication year - 2010
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2009.00959.x
Subject(s) - biology , reporter gene , promoter , transcription (linguistics) , gene , transfection , transcription factor , transcriptional regulation , microbiology and biotechnology , gene expression , regulation of gene expression , genetics , linguistics , philosophy
AeSCP‐2, a sterol carrier protein, is involved in sterol trafficking in mosquitoes. The activity of the AeSCP‐2 gene is important for mosquito development. An earlier study demonstrated that the transcription of this gene was upregulated by 20‐hydroxyecdysone (20E) in cultured gut tissues. To investigate 20E‐regulated transcription of the AeSCP‐2 gene we truncated the upstream flanking region of AeSCP‐2 gene and linked it to a reporter gene. The mosquito Aag‐2 cell line was transfected with these promoter/reporter constructs and treated with 20E at various concentrations. Expression vectors of different transcription factors such as HR3 and βFTZ‐F1 were also co‐transfected with the AeSCP‐2 promoter/reporter constructs. The observed results demonstrated that varied combinations of transcription factors produce different promoter activities of the AeSCP‐2 gene. This observation leads us to the conclusion that the partnership of transcription factors is crucial in regulating the transcriptional activity of the AeSCP‐2 gene.