Premium
A full‐length cDNA resource for the pea aphid, Acyrthosiphon pisum
Author(s) -
Shigenobu S.,
Richards S.,
Cree A. G.,
Morioka M.,
Fukatsu T.,
Kudo T.,
Miyagishima S.,
Gibbs R. A.,
Stern D. L.,
Nakabachi A.
Publication year - 2010
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2009.00946.x
Subject(s) - acyrthosiphon pisum , biology , aphid , functional genomics , gene , complementary dna , expressed sequence tag , genetics , annotation , genome , genomics , cdna library , computational biology , whole genome sequencing , gene prediction , botany , aphididae , pest analysis , homoptera
Large collections of full‐length cDNAs are important resources for genome annotation and functional genomics. We report the creation of a collection of 50 599 full‐length cDNA clones from the pea aphid, Acyrthosiphon pisum . Sequencing from 5′ and 3′ ends of the clones generated 97 828 high‐quality expressed sequence tags, representing approximately 9000 genes. These sequences were imported to AphidBase and are shown to play crucial roles in both automatic gene prediction and manual annotation. Our detailed analyses demonstrated that the full‐length cDNAs can further improve gene models and can even identify novel genes that are not included in the current version of the official gene set. This full‐length cDNA collection can be utilized for a wide variety of functional studies, serving as a community resource for the study of the functional genomics of the pea aphid.