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Developmental regulation of the pheromone biosynthesis activating neuropeptide‐receptor (PBAN‐R): re‐evaluating the role of juvenile hormone
Author(s) -
Bober R.,
Azrielli A.,
Rafaeli A.
Publication year - 2010
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2009.00937.x
Subject(s) - juvenile hormone , biology , pheromone , medicine , neuropeptide , endocrinology , receptor , sex pheromone , helicoverpa armigera , hormone , period (music) , larva , biochemistry , genetics , botany , physics , acoustics
Sex pheromone production in Helicoverpa armigera is regulated by pheromone‐biosynthesis‐activating neuropeptide (PBAN), which binds to a G‐protein coupled receptor at the pheromone gland. We demonstrate the temporal differential expression levels of the PBAN receptor (PBAN‐R) gene, reaching peak levels at a critical period of 5 h post‐eclosion. Previous studies implied a possible regulatory role for juvenile hormone (JH). We herein demonstrate that PBAN‐R expression levels increase normally when females are decapitated or head‐ligated, removing the source of JH, before peak transcript levels are reached. Similarly, sex pheromone production can be induced by PBAN in such decapitated females. These results indicate that up‐regulation, at this critical time, is not dependent on JH originating from the head. Conversely, JH injected in vivo at this critical period significantly inhibits PBAN‐R transcript levels.