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Data mining cDNAs reveals three new single stranded RNA viruses in Nasonia (Hymenoptera: Pteromalidae)
Author(s) -
Oliveira D. C. S. G.,
Hunter W. B.,
Ng J.,
Desjardins C. A.,
Dang P. M.,
Werren J. H.
Publication year - 2010
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2009.00934.x
Subject(s) - biology , pteromalidae , rna , rna polymerase , genetics , rna virus , genome , mycovirus , complementary dna , virology , gene , parasitoid , host (biology)
We report three novel small RNA viruses uncovered from cDNA libraries from parasitoid wasps in the genus Nasonia . The genome of this kind of virus is a positive‐sense single‐stranded RNA with a 3′ poly(A), which facilitates cloning from cDNAs. Two of the viruses, NvitV‐1 and NvitV‐2, possess a RNA‐dependent RNA polymerase that associates them with the family Iflaviridae of the order Picornavirales . A third virus, NvitV‐3, is most similar to the Nora virus from Drosophila . A reverse transcription‐PCR method developed for NvitV‐1 indicates that it is a persistent commensal infection of Nasonia .