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A coleopteran triosephosphate isomerase: X‐ray structure and phylogenetic impact of insect sequences
Author(s) -
Knobeloch D.,
Schmidt A.,
Scheerer P.,
Krauss N.,
Wessner H.,
Scholz Ch.,
Küttner G.,
Von Rintelen T.,
Wessel A.,
Höhne W.
Publication year - 2010
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2009.00928.x
Subject(s) - triosephosphate isomerase , biology , mealworm , phylogenetic tree , insect , phylogenetics , escherichia coli , evolutionary biology , genetics , gene , botany , larva
A coleopteran triosephosphate isomerase (TIM) from Tenebrio molitor (yellow mealworm beetle) was recombinantly expressed in Escherichia coli and characterized with respect to thermal stability, kinetic parameters and oligomeric state. The enzyme was successfully crystallized and the structure determined by X‐ray analysis to 2.0 Å resolution. This is the first example of an invertebrate TIM. We compare structural features with known structures of TIMs from microorganisms, plants and vertebrates, and discuss the utility of the Tenebrio TIM sequence, together with several newly sequenced insect TIMs, for molecular phylogenetic analysis.