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RNA interference‐mediated knock‐down of Bla g 1 in the German cockroach, Blattella germanica L., implicates this allergen‐encoding gene in digestion and nutrient absorption
Author(s) -
Suazo A.,
Gore C.,
Schal C.
Publication year - 2009
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2009.00912.x
Subject(s) - juvenile hormone , biology , german cockroach , rna interference , gene knockdown , cockroach , nicotiana benthamiana , rna , microbiology and biotechnology , gene silencing , gene , medicine , endocrinology , hormone , genetics , ecology
We used RNA interference (RNAi) to silence the expression of a gene encoding Bla g 1, a human allergen produced by the German cockroach, Blattella germanica L., to study its function in cockroach physiology. Females injected with 1 µg of double‐stranded RNA contained 64% less Bla g 1 protein and Bla g 1 mRNA abundance was reduced by 91.4% compared to sham‐injected females. Bla g 1 knockdown slowed the pace of weight gain, midgut growth, and colleterial gland and basal oocyte maturation, resulting in delayed egg case formation and lower fecundity. Exogenous juvenile hormone treatments rescued reproduction in RNAi‐treated females, suggesting that Bla g 1 silencing lowered endogenous juvenile hormone, probably by reducing food intake and nutrient absorption.