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Begomovirus coat protein interacts with a small heat‐shock protein of its transmission vector ( Bemisia tabaci )
Author(s) -
Ohnesorge S.,
Bejarano E. R.
Publication year - 2009
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2009.00906.x
Subject(s) - whitefly , biology , coat protein , begomovirus , vector (molecular biology) , plant virus , transmission (telecommunications) , two hybrid screening , botany , yeast , virology , genetics , gene , virus , rna , recombinant dna , engineering , electrical engineering
Tomato yellow leaf curl Sardinia virus (TYLCSV) is transmitted from plant to plant by the whitefly Bemisia tabaci in a persistent‐circulative manner. The coat protein (CP) plays an important role in this transmission cycle. In this study, the CP was used to screen a Bemisia tabaci cDNA library using the yeast two‐hybrid system, in a search for interacting partners. A member of the small heat‐shock protein family (termed BtHSP16) was identified and its interaction with the CP was verified by an in vitro pull‐down assay. The binding domain was located at the variable N‐terminal part of the CP, while full‐length BtHSP16 is required for the interaction. The putative role for this interaction in the transmission cycle by the whitefly is discussed.