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Cognate Hsp 70 gene is induced during deep larval diapause in the moth Sesamia nonagrioides
Author(s) -
Gkouvitsas T.,
Kontogiannatos D.,
Kourti A.
Publication year - 2009
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2009.00866.x
Subject(s) - diapause , biology , heat shock protein , hsp70 , complementary dna , gene , larva , amino acid , microbiology and biotechnology , biochemistry , botany
The complete cDNA sequences of Heat shock cognate protein 70 ( SnoHsc 70) and Heat shock protein 70 ( SnoHsp 70) were determined from the corn stalk borer Sesamia nonagrioides (Lef.). They encode 653 amino acids (Hsc70) and 633 amino acids (Hsp70), with calculated molecular masses of 71.5 kDa and 70.2 kDa respectively. SnoHsc 70 is constitutively expressed, and SnoHsp 70 is heat‐inducible in non‐diapausing insects. SnoHsp 70 is down regulated during diapause, while SnoHsc 70 is induced as the larvae enter deep diapause. High temperature stress during diapause has no further effect on transcript levels of SnoHsc 70. Our results show that SnoHsc 70 may play important roles in assisting protein conformation during specific stages of diapause.