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An inducible HSP70 gene from the midge Chironomus dilutus : characterization and transcription profile under environmental stress
Author(s) -
KarounaRenier N. K.,
Rao K. Ranga
Publication year - 2009
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2008.00853.x
Subject(s) - midge , biology , chironomus , hsp70 , gene , heat shock protein , phylogenetic tree , gene expression , transcription factor , reverse transcriptase , genetics , polymerase chain reaction , chironomidae , botany , gall , larva
In the present study, we identified and characterized an inducible heat shock protein 70 (HSP70) from the midge Chironomus dilutus and investigated the transcriptional profile of the gene under baseline and environmentally stressful conditions. Using real‐time quantitative reverse transcriptase‐polymerase chain reaction (qRT‐PCR), we observed increased expression of CD‐HSP70‐1 in response to both heat shock and copper stress. We also investigated the expression of this gene during midge development. All C. dilutus developmental stages expressed CD‐HSP70‐1 under normal conditions, although at extremely low levels. Phylogenetic analysis of the amino acid sequence demonstrated distinct clustering of this gene with inducible HSP70s from other insect species.