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Microarray analysis of acaricide‐inducible gene expression in the southern cattle tick, Rhipicephalus (Boophilus) microplus
Author(s) -
Saldivar L.,
Guerrero F. D.,
Miller R. J.,
Bendele K. G.,
Gondro C.,
Brayton K. A.
Publication year - 2008
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2008.00831.x
Subject(s) - biology , acaricide , rhipicephalus microplus , amitraz , rhipicephalus , microarray , coumaphos , gene expression , gene , microarray analysis techniques , tick , ivermectin , dna microarray , expressed sequence tag , permethrin , microbiology and biotechnology , genetics , virology , pesticide , toxicology , zoology , agronomy
Acaricide‐inducible differential gene expression was studied in larvae of Rhipicephalus (Boophilus) microplus using a microarray‐based approach. The acaricides used were: coumaphos, permethrin, ivermectin, and amitraz. The microarrays contained over 13 000 probes, having been derived from a previously described R. microplus gene index (BmiGI Version 2; Wang et al ., 2007). Relative quantitative reverse transcriptase‐PCR, real time PCR, and serial analysis of gene expression data was used to verify microarray data. Among the differentially expressed genes with informative annotation were legumain, glutathione S‐transferase, and a putative salivary gland‐associated protein.