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Inducible‐ and constitutive‐type transcript variants of kakusei , a novel non‐coding immediate early gene, in the honeybee brain
Author(s) -
Kiya Taketoshi,
Kunieda Takekazu,
Kubo Takeo
Publication year - 2008
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2008.00821.x
Subject(s) - biology , gene , genetics , rna , messenger rna , locus (genetics) , coding region , gene expression , non coding rna , microbiology and biotechnology
We previously identified a novel non‐coding immediate early gene, termed kakusei , from the honeybee ( Apis mellifera ) and used it as a marker to detect neural activity in the brains of foraging workers (Kiya et al ., 2007). Here, we investigated the detailed kakusei gene structure. Expression analysis revealed that, in addition to the neural activity‐inducible transcript variant, multiple neural activity‐independent transcript variants were constitutively expressed from the same kakusei locus. In situ hybridization revealed that constitutive‐type kakusei variants were detected in the whole brain and the RNA was localized predominantly in the neural nuclei, like the inducible‐type variant, suggesting the concerted action of inducible‐ and constitutive‐types of kakusei transcript variants on nuclear function.