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The cloning and expression of α‐tubulin in Monochamus alternatus
Author(s) -
Song L.,
Liu X.X.,
Zhang Y.A.,
Zhang Q.W.,
Zhao Z.W.
Publication year - 2008
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2008.00818.x
Subject(s) - monochamus alternatus , biology , tubulin , bursaphelenchus xylophilus , cloning (programming) , gene , homology (biology) , escherichia coli , western blot , botany , nematode , microbiology and biotechnology , genetics , ecology , microtubule , longhorn beetle , computer science , programming language
The Japanese pine sawyer Monochamus alternatus is one of the major forest pests. It damages pine directly and transfers the nematode Bursaphelenchus xylophilus to pine wood; resulting in serious economic losses around the world every year. α‐tubulin is one of most important proteins in most species. We cloned a ubiquitously expressed M. alternatus α‐tubulin gene and analysed its nucleotides and protein structure; its sequence characters are consistent with what have been reported in other insects. The alignment of proteins showed that there is high homology of α‐tubulin between M. alternatus and other species. Western blot and immunocytochemistry analyses suggested a common epitope of α‐tubulin between M. alternatus and Strongylcentrotus purpuratus . We also expressed the protein in Escherichia coli for further functional studies.