Premium
Expressed sequence tags and proteomics of antennae from the tortricid moth, Epiphyas postvittana
Author(s) -
Jordan M. D.,
Stanley D.,
Marshall S. D. G.,
De Silva D.,
Crowhurst R. N.,
Gleave A. P.,
Greenwood D. R.,
Newcomb R. D.
Publication year - 2008
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2008.00812.x
Subject(s) - biology , tortricidae , proteome , pheromone , sex pheromone , proteomics , gene , lepidoptera genitalia , genetics , botany
Genomic and proteomic analyses of the antennae of the light brown apple moth, Epiphyas postvittana (Walker) (Lepidoptera: Tortricidae) were undertaken to identify genes and proteins potentially involved in odorant and pheromone binding and turnover. An EST approach yielded 5739 sequences, comprising 808 contigs and 1545 singletons. InterPro and B last analyses revealed members of families implicated in odorant and pheromone binding (PBPs, GOBPs, ABPXs and CSPs) and turnover (CXEs, GSTs, CYPs). Of the three pheromone binding proteins (PBPs) identified, two were more highly expressed at the RNA and protein levels in adult male antennae (EpPBP1, EpPBP3), while a third was more highly expressed in female antennae (EpPBP2). To identify proteins involved in the detection of sex‐specific signals, differential 2D gel electrophoresis (pH 5–8) followed by mass spectrometry was conducted on antennal proteins from males versus females. Identified male‐biased proteins included a pheromone binding protein, a porin, a short chain dehydrogenase/reductase, and a member of the takeout family.