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Cloning and molecular characterization of two invertebrate‐type lysozymes from Anopheles gambiae
Author(s) -
Paskewitz S. M.,
Li B.,
Kajla M. K.
Publication year - 2008
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2008.00797.x
Subject(s) - biology , anopheles gambiae , malpighian tubule system , cloning (programming) , gene , anopheles , phylogenetic tree , insect , blood meal , genetics , gene expression , sequence analysis , microbiology and biotechnology , zoology , midgut , botany , larva , malaria , immunology , computer science , programming language
We sequenced and characterized two novel invertebrate‐type lysozymes from the mosquito Anopheles gambiae . Alignment and phylogenetic analysis of these and a number of related insect proteins identified through bioinformatics strategies showed a high degree of conservation of this protein family throughout the Class Insecta. Expression profiles were examined for the two mosquito genes through semiquantitative and real‐time PCR analysis. Lys i‐1 transcripts were found in adult females in the fat body and Malpighian tubules, whereas Lys i‐2 was detected only in fat bodies. Blood‐feeding resulted in significantly increased transcript abundance for both genes in the midguts. Neither gene was upregulated following bacterial challenge.