Premium
Regulation of Junonia coenia densovirus P9 promoter expression
Author(s) -
Shirk P. D.,
Bossin H.,
Furlong R. B.,
Gillett J. L.
Publication year - 2007
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2007.00759.x
Subject(s) - biology , enhancer , transformation (genetics) , transcription factor , capsid , microbiology and biotechnology , genetics , gene , transcription (linguistics) , promoter , gene expression , linguistics , philosophy
Transcriptional activity of the Junonia coenia densovirus ( Jc DNV) P9 promoter depends on a 557‐bp sequence located within the overlapping 3′ sequences for viral capsid and nonstructural genes. Utilizing a somatic transformation assay to assess Jc DNV promoter activity in Drosophila melanogaster and Plodia interpunctella , viral sequences were subjected to deletional analysis. Removal of a 685‐bp fragment reduced P9‐driven expression to background levels. Inclusion of a second expression cassette demonstrated vector persistence and confirmed somatic transformation. P9 promoter‐driven expression was restored by insertion of a 557‐bp Jc DNV fragment or by inclusion of a heterologous baculovirus hr5 enhancer. Consensus polycomb transcriptional factor binding sites were identified within the 557‐bp fragment, which suggests a potential role in regulating densoviral transcription.