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Changes in Drosophila melanogaster midgut proteins in response to dietary Bowman–Birk inhibitor
Author(s) -
Li H.M.,
Margam V.,
Muir W. M.,
Murdock L. L.,
Pittendrigh B. R.
Publication year - 2007
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2007.00750.x
Subject(s) - biology , drosophila melanogaster , midgut , proteome , biochemistry , f box protein , catabolism , enzyme , ubiquitin , microbiology and biotechnology , gene , larva , ubiquitin ligase , botany
The midgut proteome of Drosophila melanogaster was compared in larvae fed dietary Bowman–Birk inhibitor (BBI) vs. larvae fed a control diet. By using two‐dimensional gel electrophoresis, nine differentially expressed proteins were observed, which were associated with enzymes or transport functions such as sterol carrier protein X (SCPX), ubiquitin‐conjugating enzyme, endopeptidase, receptor signalling protein kinase, ATP‐dependent RNA helicase and α‐tocopherol transport. Quantitative real‐time PCR verified differential expression of transcripts coding for six of the proteins observed from the proteomic analysis. BBI evidently affects expression of proteins associated with protein degradation, transport and fatty acid catabolism. We then tested the hypothesis that SCPX was critical for the Drosophila third instars’ response to BBI treatment. Inhibition of SCPX caused the third instars to become more susceptible to dietary BBI.