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Evaluation of the role of CYP6B cytochrome P450s in pyrethroid resistant Australian Helicoverpa armigera
Author(s) -
Grubor Vladimir D.,
Heckel David G.
Publication year - 2007
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2006.00697.x
Subject(s) - helicoverpa armigera , pyrethroid , biology , genetics , gene , fenvalerate , cytochrome p450 , amplified fragment length polymorphism , cypermethrin , genotype , genome , lepidoptera genitalia , botany , pesticide , enzyme , agronomy , biochemistry , population , genetic diversity , demography , sociology
The AN02 strain of Helicoverpa armigera from eastern Australia exhibits 50‐fold, PBO‐suppressible resistance to the pyrethroid insecticide fenvalerate. The semidominant resistance gene RFen1 was previously mapped to AFLP Linkage Group 13. In evaluating the cytochrome P450 genes CYP6B7 , CYP6B6 , and CYP6B2 as candidates for RFen1 , we found that they occur in a tandem array in the genome, next to the gene encoding the para ‐type sodium channel; the target of pyrethroid insecticides. We mapped these genes to AFLP Linkage Group 14, thus rejecting mutations within the P450 cluster or para as candidates for RFen1 . RFen1 genotypes produced slightly different mRNA levels of the three P450s, but the differences were too small to convincingly account for resistance. We conclude that even if one or more of these P450s metabolize fenvalerate, they are unlikely to be responsible for the resistance in AN02.