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Methionine‐rich storage protein gene in the rice stem borer, Chilo suppressalis , is expressed during diapause in response to cold acclimation
Author(s) -
Sonoda S.,
Fukumoto K.,
Izumi Y.,
Ashfaq M.,
Yoshida H.,
Tsumuki H.
Publication year - 2006
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2006.00670.x
Subject(s) - chilo suppressalis , diapause , biology , methionine , complementary dna , gene , acclimatization , gene expression , microbiology and biotechnology , storage protein , larva , botany , biochemistry , amino acid
Gene expressions of acclimatized and non‐acclimatized diapausing larvae were examined in Chilo suppressalis using a subtraction technique. A gene encoding a methionine‐rich storage protein, CsSP1 , was cloned and its complete cDNA sequence was determined. Potentially, CsSP1 encoded a 758‐amino acid protein, with a calculated molecular weight of 88.8 kDa. The expression level of CsSP1 was higher in nondiapausing larvae than in diapausing ones. The CsSP1 expression was up‐regulated in diapausing larvae when the temperature of cold acclimation was shifted to 5 °C. The up‐regulated level was maintained at 40 days after incubation at 5 °C. In nondiapausing larvae, CsSP1 expression was down‐regulated when the temperature was below developmental zero. Involvement of CsSP1 in diapause, cold tolerance acquisition and postdiapause development in C. suppressalis is discussed.