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Genomic and transcriptional analysis of protein heterogeneity of the honeybee venom allergen Api m 6
Author(s) -
Peiren N.,
De Graaf D. C.,
Evans J. D.,
Jacobs F. J.
Publication year - 2006
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2006.00669.x
Subject(s) - biology , indel , genome , genetics , gene , venom , locus (genetics) , untranslated region , exon , coding region , genetic variation , protein sequencing , single nucleotide polymorphism , peptide sequence , messenger rna , genotype , ecology
Several components of honeybee venom are known to cause allergenic responses in humans and other vertebrates. One such component, the minor allergen Api m 6, has been known to show amino acid variation but the genetic mechanism for this variation is unknown. Here we show that Api m 6 is derived from a single locus, and that substantial protein‐level variation has a simple genome‐level cause, without the need to invoke multiple loci or alternatively spliced exons. Api m 6 sits near a misassembled section of the honeybee genome sequence, and we propose that a substantial number of indels at and near Api m 6 might be the root cause of this misassembly. We suggest that genes such as Api m 6 with coding‐region or untranslated region indels might have had a strong effect on the assembly of this draft of the honeybee genome.