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Transcriptional response of Choristoneura fumiferana to sublethal exposure of Cry1Ab protoxin from Bacillus thuringiensis
Author(s) -
Meunier L.,
Préfontaine G.,
Van Munster M.,
Brousseau R.,
Masson L.
Publication year - 2006
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2006.00659.x
Subject(s) - bacillus thuringiensis , biology , choristoneura fumiferana , gene , larva , lepidoptera genitalia , botany , bacteria , spruce budworm , genetics , tortricidae
Bacillus thuringiensis is a microbial control agent active against Choristoneura fumiferana , a lepidopteran defoliator of North American forests. Although the B. thuringiensis insecticidal crystal protoxins have a relatively narrow host range, there is concern about their impact on non‐target species where intoxication effects may not be overt. Larval toxicity effects can be assessed at the molecular level by determining altered transcriptional profiles in response to sublethal protoxin exposure in sensitive insects. Subtraction hybridization libraries were created using two larval populations, control and protoxin‐fed and were characterized by sequencing 1091 clones. Differential mRNA expression of selected clones, as measured by quantitative polymerase chain reaction, identified a number of metabolic and stress‐related genes that were either transcriptionally enhanced or repressed after protoxin exposure.