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Anopheles gambiae P450 reductase is highly expressed in oenocytes and in vivo knockdown increases permethrin susceptibility
Author(s) -
Lycett G. J.,
McLaughlin L. A.,
Ranson H.,
Hemingway J.,
Kafatos F. C.,
Loukeris T. G.,
Paine M. J. I.
Publication year - 2006
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2006.00647.x
Subject(s) - gene knockdown , biology , anopheles gambiae , drosophila melanogaster , cytochrome p450 , rna interference , permethrin , pyrethroid , microbiology and biotechnology , knockdown resistance , gene , genetics , metabolism , rna , malaria , biochemistry , immunology , pesticide , ecology , cyfluthrin
We describe an in vivo model for investigation of detoxification mechanisms of the mosquito Anopheles gambiae , important for the development of malaria control programmes. Cytochrome P450s are involved in metabolic insecticide resistance and require NADPH cytochrome P450 reductase (CPR) to function. Here we demonstrate that the major sites of adult mosquito CPR expression are oenocytes, mid‐gut epithelia and head appendages. High CPR expression was also evident in Drosophila oenocytes indicating a general functional role in these insect cells. RNAi mediated knockdown drastically reduced CPR expression in oenocytes, and to a lesser extent in mid‐gut epithelia; the head was unaffected. These flies showed enhanced sensitivity to permethrin, demonstrating a key role for abdominal/mid‐gut P450s in pyrethroid metabolism, aiding the development of insecticides.