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Cloning and characterization of the homologous genes of firefly luciferase in the mealworm beetle, Tenebrio molitor
Author(s) -
Oba Y.,
Sato M.,
Inouye S.
Publication year - 2006
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2006.00646.x
Subject(s) - mealworm , luciferase , biology , gene , biochemistry , firefly protocol , insect , cloning (programming) , lampyridae , genetics , botany , larva , zoology , transfection , computer science , programming language
Three homologous genes of firefly luciferase were cloned from the non‐luminous beetle Tenebrio molitor . Three gene products for homologues, TmLL‐1, TmLL‐2 and TmLL‐3, showed fatty acyl‐coenzyme A (acyl‐CoA) synthetic activity, but not luciferase activity with firefly luciferin. The transcripts were detected through the developmental stages in T. molitor . These results suggested that firefly luciferase was evolved from a fatty acyl‐coenzyme A synthetase by gene duplications in the insect.