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Germ line transformation of the olive fly Bactrocera oleae using a versatile transgenesis marker
Author(s) -
Koukidou M.,
Klinakis A.,
Reboulakis C.,
Zagoraiou L.,
Tavernarakis N.,
Livadaras I.,
Economopoulos A.,
Savakis C.
Publication year - 2006
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2006.00613.x
Subject(s) - transposase , biology , transgenesis , bactrocera , transformation (genetics) , genetics , transposable element , gene , pest analysis , botany , mutant , tephritidae , reproductive technology , embryogenesis
The olive fruit fly (olive fly) Bactrocera oleae (Dacus), recently introduced in North America, is the most destructive pest of olives worldwide. The lack of an efficient gene transfer technology for olive fly has hampered molecular analysis, as well as development of genetic techniques for its control. We have developed a Minos ‐based transposon vector carrying a self‐activating cassette which overexpresses the enhanced green fluorescent protein (EGFP). Efficient transposase‐mediated integration of one to multiple copies of this vector was achieved in the germ line of B. oleae by coinjecting the vector along with in vitro synthesized Minos transposase mRNA into preblastoderm embryos. The self‐activating gene construct combined with transposase mRNA present a system with potential for transgenesis of very diverse species.