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Gene structure and expression profile of Manduca sexta prophenoloxidase‐activating proteinase‐3 (PAP‐3), an immune protein containing two clip domains
Author(s) -
Zou Z.,
Jiang H.
Publication year - 2005
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2005.00574.x
Subject(s) - prophenoloxidase , manduca sexta , biology , exon , gene , gene expression , manduca , microbiology and biotechnology , alternative splicing , genetics , immune system , innate immune system , botany , larva
Prophenoloxidase‐activating proteinase‐3 (PAP‐3) is a component of the defence system in Manduca sexta . We have isolated genomic clones and elucidated the organization of this gene. The 3′ end of exon 2, the entire exon 3 and the 5′ end of exon 4 encode the two amino‐terminal clip domains. Southern blot analysis suggested a single copy of the PAP‐3 gene in the genome. We identified several putative immune‐responsive elements in the upstream region. The PAP‐3 gene is not highly expressed in the fat body during larval development until the wandering stage begins. The mRNA level is high in the epithelium, fat body and haemocytes. Tissue‐specific alternative splicing occurs in the fat body and trachea. A bacterial injection markedly induced the gene expression in the fat body and haemocytes.