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A group of related cDNAs encoding secreted proteins from Hessian fly [ Mayetiola destructor (Say)] salivary glands
Author(s) -
Chen M.S.,
Fellers J. P.,
Stuart J. J.,
Reese J. C.,
Liu X.
Publication year - 2004
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2004.00465.x
Subject(s) - biology , hessian matrix , destructor , salivary gland , microbiology and biotechnology , botany , biochemistry , mite , mathematics
A group of cDNAs has been isolated and characterized from Hessian fly [ Mayetiola destructor (Say)] salivary glands. Members in this group appear to encode proteins with secretion signal peptides at the N‐terminals. The mature putative proteins are small, basic proteins with calculated molecular weights that ranged from 8.5 to 10 kDa, and isoelectric points from 9.92 to 10.90. Sequence analysis indicated a strong selection for mutations that generate amino acid changes within the coding region. Northern blot analysis revealed that these genes are expressed only in the first instar larvae, a critical stage that determines if the interaction between a specific Hessian fly biotype and a specific wheat cultivar is compatible. Genomic analysis demonstrated that multiple copies of similar genes are clustered within a short region on chromosome 2A. This is the same arm in which two avirulence genes have been mapped.