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Molecular cloning and functional expression of a serotonin receptor from the Southern cattle tick, Boophilus microplus (Acari: Ixodidae)
Author(s) -
Chen A.,
Holmes S. P.,
Pietrantonio P. V.
Publication year - 2004
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2004.00457.x
Subject(s) - biology , receptor , complementary dna , microbiology and biotechnology , 5 ht receptor , tick , g protein coupled receptor , 5 ht5a receptor , ixodidae , molecular cloning , acari , serotonin , gene , genetics , virology , botany
A full‐length cDNA encoding a 5‐hydroxytryptamine (5‐HT) receptor from the Southern cattle tick, Boophilus microplus , was isolated using a strategy based on sequence homology among G protein‐coupled receptors. The deduced amino acid sequence revealed highest identity with Drosophila melanogaster 5HT‐dro2A (Z11489, 50.8%) and 5HT‐dro2B (Z11490, 49.5%) receptors. The receptor was transiently expressed in mammalian HEK293 cells, and Western blot analysis showed the expected 43.3 kDa band. In these cells, application of 5‐HT (10 µ m ) inhibited forskolin‐induced cAMP synthesis by 26%. The results indicate that the tick receptor is an invertebrate 5‐HT1‐like receptor that couples to G αi protein.