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Identification of phytoplasmas in eggs, nymphs and adults of Scaphoideus titanus Ball reared on healthy plants
Author(s) -
Alma A.,
Bosco D.,
Danielli A.,
Bertaccini A.,
Vibio M.,
Arzone A.
Publication year - 1997
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.1997.tb00079.x
Subject(s) - biology , phytoplasma , nymph , aster yellows , leafhopper , vicia faba , instar , transovarial transmission , botany , nested polymerase chain reaction , horticulture , veterinary medicine , restriction fragment length polymorphism , hemiptera , polymerase chain reaction , larva , genetics , medicine , gene
A survey for the presence of aster yellows‐related phytoplasmas in the different life stages of Scaphoideus titanus was carried out by means of PCR and nested‐PCR assays. Using a phytoplasma 16Srl group‐specific primer pair followed by RFLP analysis of amplified products, we were able to detect and identify phytoplasmas from eggs, newly hatched nymphs, fourth and fifth instar nymphs and adults reared on ten phytoplasma‐free Vicia faba seedlings. Two of these broad beans became infected, whereas PCR failed to detect phytoplasmas in the same ten plants before leafhopper rearing. These results suggest the possibility of transovarial transmission of aster yellows‐related phytoplasmas in S. titanus .