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The structure, sequence and developmental pattern of expression of the white gene in the blowfly Lucilia cuprina
Author(s) -
Garcia R. L.,
Perkins H. D.,
Howells A. J.
Publication year - 1996
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.1996.tb00099.x
Subject(s) - lucilia cuprina , biology , gene , white (mutation) , genetics , drosophila melanogaster , coding region , intron , exon , melanogaster , botany , larva , calliphoridae
We have sequenced the complete coding region of the white gene of Lucilia cuprina . Strong sequence identity exists between this gene and its homologue from Drosophila melanogaster at both nucleotide and derived amino acid levels (68% and 78% respectively). The exon/intron structure of the two genes is also largely conserved, although the Lucilia gene contains one extra Won. Expression of the gene peaks during mid‐pupal stage, with secondary peaks in late larval and early adult stages. Comparisons between this and other white genes will contribute to a better understanding of ATP‐binding transmembrane transport proteins. The white gene should also serve as a useful marker gene in the development of a gene transformation system for the sheep blowfly.