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Isolation of a cluster esterase genes associated with organophosphate resistance in Lucilia cuprina
Author(s) -
Newcomb R. D.,
East P. D.,
Russell R. J.,
Oakeshott J. G.
Publication year - 1996
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.1996.tb00056.x
Subject(s) - lucilia cuprina , esterase , biology , melanogaster , gene , organophosphate , drosophila melanogaster , genetics , gene cluster , midgut , structural gene , enzyme , biochemistry , calliphoridae , pesticide , botany , escherichia coli , ecology , larva
PCR primers designed from the α‐esterase gene cluster of Drosophiia melanogaster have been used to isolate fragments from eight esterase genes In the Australian sheep blowfly, Lucilia cuprina . Phyio‐genetic analysis suggests that three are homologues of the αE7, αE8 and αE9 genes of the α‐esterase cluster of D. melanogaster . A further three are also probably α‐esterases, whereas the remaining two more closely resemble β‐esterases. Transcripts for five of the α‐esterase genes were detected by PCR in adult midgut, consistent with a role for these enzymes in digestion andlor detoxification. Based on the tissue distribution of these transcripts, LcαE7 may possibly encode the esterase, E3, which is involved in organophosphate resistance.