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Analysis of mosquito genome structure using graphical genotyping
Author(s) -
Severson D. W.,
Kassner V. A.
Publication year - 1995
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.1995.tb00033.x
Subject(s) - genotyping , biology , genome , genetics , restriction fragment length polymorphism , genotype , locus (genetics) , chromosome , computational biology , gene
Restriction fragment length polymorphism (RFLP) markers provide effective tools for establishing the genotype at many loci for individual mosquitoes. RFLP genotypes can be organized to provide information concerning parental origin or the allelic configuration of various chromosome segments. Whole genome genotypic information for individuals can be represented in a simple graphic format, termed graphical genotyping. In this study, RFLP markers were used to demonstrate the utility of using graphical genotyping to easily and quickly evaluate the entire genome of the mosquito Aedes aegypti. Two substrains selected for susceptibility and refractoriness, from a strain highly susceptible to the filarial worm parasite Brugia malayi , were compared. Graphical genotyping provided a mechanism to rapidly evaluate genome structure both within and between the two substrains with a simple visual analysis. This information was used in related studies to identify a new locus influencing B. malayi susceptibility in A. aegypti. Graphical genotyping also provided perspectives on some features of genome structure in A. aegypti. It suggested the presence and location of a genetic lethal on chromosome 3 and possible inversion polymorphisms associated with each of the three A. aegypti chromosomes.