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Replication and expression of a recombinant Sindbis virus in mosquitoes
Author(s) -
RaymsKeller A.,
Powers A. M.,
Higgs S.,
Olson K. E.,
Kamrud K. I.,
Carlson J. O.,
Beaty B. J.
Publication year - 1995
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.1995.tb00030.x
Subject(s) - sindbis virus , biology , virology , virus , titer , alphavirus , viral replication , recombinant dna , togaviridae , vector (molecular biology) , recombinant virus , rna , aedes , aedes aegypti , microbiology and biotechnology , gene , dengue fever , botany , larva , biochemistry
A recombinant Sindbis virus, TE/3'2J/ANTI‐S, containing LaCrosse virus small segment cDNA in antisense orientation, was inoculated into Aedes triseriatus mosquitoes. Virus replication and LAC‐ANTI‐S RNA expression were analysed temporally and spatially. TE/3'2J/ANTI‐S virus titre peaked at 5.0 log 10 TCID 50 in heads 6–9 days post infection (p.i.) and decreased to 3.4 log 10 TCID 50 by 37 days p.i. Salivary glands contained 4.4 log 10 TCID 50 of virus 6 days p.i.; titres were lower in other organs. LAC‐ANTI‐S RNA levels paralleled virus titre. SIN E1 antigen was detected in many mosquito organ systems, but in specific cells and tissues of some organs. TE/3'2J/ANTI‐S virus exhibited different cellular tropisms in salivary glands of Aedes and Culex mosquitoes.