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Primary sequence, copy number, and distribution of mariner transposons in the honey bee
Author(s) -
Ebert P. R.,
Hileman J. P.,
Nguyen H. T.
Publication year - 1995
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.1995.tb00010.x
Subject(s) - transposable element , biology , genome , inverted repeat , genetics , gene duplication , southern blot , transposase , repeated sequence , whole genome sequencing , gene
Abstract A single honey bee mariner transposon (TnM1a) was sequenced, revealing a transpositionally non‐autonomous element of 937 bp delimited by 30 bp perfect inverted terminal repeats. The element is flanked by the TA duplication typical of mariner elements in general. There are approximately 435 copies of TnM1a homologous elements per haploid genome. These elements appear, by Southern blot analysis, to be dispersed throughout the genome. Thirteen individual genomic clones with an average size of 15 kb, were found to contain only a single element each, which also suggests that the elements are not tightly clustered. Finally, mariner elements are neither inactivated by methylation nor sequestered into a methylated fraction of the genome.