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High‐level expression of the bacterial opd gene in Drosophila melanogasten improved inducible insecticide resistance
Author(s) -
Benedict M. Q.,
Scott J. A.,
Cockburn A. F.
Publication year - 1994
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.1994.tb00173.x
Subject(s) - biology , paraoxon , gene , gene expression , drosophila melanogaster , microbiology and biotechnology , genetics , enzyme , biochemistry , acetylcholinesterase
The bacterial parathion hydrolase gene (opd) was expressed in transformed D. melanogaster under the control of an hsp70 promoter. Transformed lines carrying chimaeric genes designed for either cytoplasmic or secretory expression exhibited high‐ or low‐level heat‐shock‐inducible transient resistance to paraoxon respectively. Greatest levels of resistance occurred approximately 12–16 h after heat shock and well after periods of maximal transcription. Insecticide resistance conferred by the cytoplasmic form of opd is expressed as a semidominant trait.