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Genome size and organization in the ixodid tick Amblyomma americanum (L.)
Author(s) -
Palmer M. J.,
Bantle J. A.,
Guo Xiaoping,
Fargoxy W. Scott
Publication year - 1994
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.1994.tb00151.x
Subject(s) - amblyomma americanum , genome , biology , genome size , tick , repeated sequence , arthropod , genomic organization , dna , dna sequencing , genetics , base pair , evolutionary biology , zoology , gene , ixodidae , virology , ecology
We used DNA reassociation kinetics to determine genome size and organization in the ixodid tick Amblyomma americanum. We calculated the genome size of A. americanum to be approximately 1.08 pg or 1.04 × 10 9 base pairs and to consist of 35.8% unique DNA, 4.2% foldback sequences, 17.9% highly repetitive sequences, and 42.1% moderately repetitive sequences. Comparison of the reassociation kinetics of long and short fragments revealed repetitive sequences to be distributed in a pattern of long period interspersion, a feature that, to date, has been associated with arthropod genomes that lack a high percentage of repetitive DNA.