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Baculovirus immediate early gene promoter based expression vectors for transient and stable transformation of insect cells
Author(s) -
Vulsteke V.,
Janssen I.,
Broeck J. Vanden,
Loot A. De,
Huybrechts R.
Publication year - 1994
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.1994.tb00139.x
Subject(s) - biology , plasmid , transformation (genetics) , microbiology and biotechnology , schneider 2 cells , gene , transfection , vector (molecular biology) , recombinant dna , drosophila melanogaster , southern blot , rna interference , genetics , rna
A recombinant plasmid vector was constructed in which the bacterial LacZ gene was placed under the control of a Bombyx mori baculovirus early promoter. The vector proved to be active in transfected cultured dipteran and lepidopteran cells. Co‐transfection carried out with this recombinant plasmid vector and a plasmid containing the hygromycin phosphotransferase gene followed by selection with the antibiotic hygromycin B, resulted in stable transformation of cultured Drosophila melanogaster Schneider 2 cells. Southern blot analysis of the host cell's genomic DNA in combination with chromosomal in situ hybridization demonstrated that multiple copies of both plasmids were integrated in the host cell's genome.