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An improved primer for PCR amplification of mitochondrial DNA in a variety of insect species
Author(s) -
Roehrdanz R. L.
Publication year - 1993
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.1993.tb00129.x
Subject(s) - biology , primer (cosmetics) , mitochondrial dna , insect , gene , polymerase chain reaction , genetics , hymenoptera , botany , chemistry , organic chemistry
A PCR primer from the mitochondrial COi gene is described that enhances the amplification of the COI‐COJI region of insect mtDNA. When used in conjunction with a primer from the COII gene identified by R. Crozier, a 1600–1700 bp segment is amplified in nine species of insects representing the orders Lepidop‐tera, Diptera, Coleoptera and Hymenoptera.