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Transfection of cultured cells of the cotton boll weevil, Anthonomus grandis , with a heat‐shock‐promoter‐chloramphenicol‐acetyltransferase construct *
Author(s) -
Stiles B.,
Heilmann J.,
Sparks R. B.,
Santoso A.,
Leopold R. A.
Publication year - 1992
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.1993.tb00108.x
Subject(s) - chloramphenicol acetyltransferase , anthonomus , transfection , biology , cauliflower mosaic virus , polylysine , microbiology and biotechnology , cell culture , boll weevil , electroporation , acetyltransferase , chloramphenicol , gene expression , reporter gene , gene , biochemistry , acetylation , transgene , genetically modified crops , genetics , botany , antibiotics
Expression of heat shock proteins (hsp) in the BRL‐AG‐3C cell line from the cotton boll weevil was examined. It was determined that the maximal expression of endogenous hsp occurred at 41°C. Various transfection methods were then compared using this cell line in conjunction with a transiently expressed bacterial gene marker (chloramphenicol acetyltransferase) which was under the control of the Drosophila hsp 70 gene promoter. The cationic lipid preparation Lipofectin was found to be very efficient at transfecting the boll weevil cells. Polylysine and 20‐hydroxyecdysone‐conjugated polylysine were moderately effective, whereas polybrene and electroporation, under the conditions reported herein, were ineffective at transfecting this cell line.