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Sequence analysis of a mosquito ribosomal protein rpL8 gene and its upstream regulatory region
Author(s) -
Lan Que,
Fallon A. M.
Publication year - 1992
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.1993.tb00107.x
Subject(s) - biology , intron , start codon , exon , genetics , gene , primer extension , microbiology and biotechnology , ribosomal protein , coding region , primer (cosmetics) , nucleic acid sequence , translational frameshift , untranslated region , ribosomal binding site , five prime untranslated region , nucleotide , ribosome , messenger rna , rna , chemistry , organic chemistry , frameshift mutation
The gene encoding Aedes albopictus ribosomal protein L8 was isolated using a cDNA probe. Based on the deduced amino acid sequence, rpL8 has a mass of 28,605 Da, a pl of 11.97, and contains 9.6% Arg and 11.9% Lys. The rpL8 gene spans 1229 nucleotides, and contains three exons measuring 73, 150, and 648 nucleotides. The first intron is 293 nucleotides long and interrupts an 85‐nucleotide untranslated leader sequence. The AUG codon is located 12 nucleotides downstream of the 5′‐end of the second exon. Separating the second and third exons is a 65‐nucleotide intron. The major transcription initiation site, identified by primer extension and polymerase stop reactions, mapped 378 nucleotides upstream from the AUG start codon; minor initiation sites were also detected. The DNA sequence upstream of the rpL8 gene was T‐rich, but conventional TATA and CAAT boxes were absent. This is the first molecular analysis of a mosquito ribosomal protein gene.