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Gene structure of Bombyx mori larval serum protein (BmLSP)
Author(s) -
Fujiwara Y.,
Yamashita O.
Publication year - 1992
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.1993.tb00106.x
Subject(s) - biology , gene , bombyx mori , genetics , intron , primer extension , bombyx , genomic dna , genomic library , transcription (linguistics) , genome , microbiology and biotechnology , peptide sequence , rna , linguistics , philosophy
To understand the molecular mechanisms of the larval‐specific transcription of Bombyx mori larval serum protein (BmLSP), we isolated a clone of the BmLSP gene from a genomic library and sequenced a 3.5‐kb fragment. An intron was found in the 5′ noncoding region of the BmLSP gene. A putative transcription start point was determined by primer extension analysis. Genomic Southern hybridization showed that there is one copy of the BmLSP gene in a haploid genome. A database search revealed that the BmLSP gene has presumptive repetitive sequences found in other B. mori genes, the sequence homologous to ecdysone‐responsive elements and a heptamer sequence found in storage protein genes.