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A Drosophila metallothionein promoter is inducible in mosquito cells
Author(s) -
Kovach M. J.,
Carlson J. O.,
Beaty B. J.
Publication year - 1992
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.1993.tb00075.x
Subject(s) - biology , plasmid , metallothionein , microbiology and biotechnology , escherichia coli , recombinant dna , gene , promoter , transcription (linguistics) , heterologous , schneider 2 cells , gene expression , genetics , rna interference , rna , philosophy , linguistics
Expression from a Drosophila metallothionein promoter ( Mtn ) was investigated in mosquito cells. Recombinant plasmids carrying a transcription unit comprised of the Escherichia coli β‐galactosidase gene ( lacZ ) fused to the metallothionein promoter were stably introduced into Aedes albopictus C6/36 cells. A low copy transformant containing approximately 60 copies of plasmid per cell, and a high copy transformant (1–2 times 10 4 copies/cell) were characterized. The expression of β‐galactosidase from the metallothionein promoter could be induced and controlled in this heterologous system, even when the copy number of introduced plasmid was several thousand.