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Natural killer T cells suppress zymosan A‐mediated granuloma formation in the liver by modulating interferon‐γ and interleukin‐10
Author(s) -
Kobayashi Takahiro,
Kawamura Hiroki,
Kanda Yasuhiro,
Matsumoto Hiroaki,
Saito Suguru,
Takeda Kazuyoshi,
Kawamura Toshihiko,
Abo Toru
Publication year - 2012
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/j.1365-2567.2012.03562.x
Subject(s) - zymosan , natural killer t cell , cd1d , granuloma formation , immunology , granuloma , biology , interferon , interferon gamma , interleukin 12 , cytokine , t cell , chemistry , immune system , cytotoxic t cell , biochemistry , in vitro
Summary Wild‐type (WT) and CD1d −/− [without natural killer (NK) T cells] mice were treated with zymosan A to induce granuloma formation in the liver. Increased granuloma formation was seen in NKT‐less mice on days 7 and 14 after administration. WT mice showed limited granuloma formation, and zymosan A eventually induced NKT cell accumulation as identified by their surface marker (e.g. CD1d‐tetramer). Zymosan A augmented the expression of Toll‐like receptor 2 on the cell surface of both macrophages and NKT cells. One possible reason for accelerated granuloma formation in NKT‐less mice was increased production of interferon‐ γ (IFN‐γ); a theory that was confirmed using IFN‐γ −/− mice. Also, zymosan A increased interleukin‐10 production in WT mice, which suppresses IFN‐γ production. Taken together, these results suggest that NKT cells in the liver have the potential to suppress zymosan A‐mediated granuloma formation.